A Study Of Inheritable Traits in Fruit Flies

A Study Of Inheritable Traits in Fruit Flies
INTRODUCTION
The Drosophila melanogaster, more commonly known as the fruit fly, is a
popular species used in genetic experiments. In fact, Thomas Hunt Morgan began
using Drosophila in the early 1900s to study genes and their relation to
certain chromosomes(Biology 263). Scientists have located over 500 genes on the
four chromosomes in the fly. There are many advantages in using Drosophila for
these types of studies. Drosophila melanogaster can lay hundreds of eggs after
just one mating, and have a generation time of two weeks at 21C(Genetics:
Drosophila Crosses 9). Another reason for using fruit flies is that they mature
rather quickly and dont require very much space. Drosophila melanogaster has a
life cycle of four specific stages. The first stage is the egg, which is about .

5mm long. In the 24 hours when the fly is in the egg stage, numerous cleavage
nuclei form. Next, the egg hatches to reveal the larva. During this stage,
growth and molting occur. Once growth is complete, the Drosophila enter the
pupal stage, where it develops into an adult through metamorphosis. Upon
reaching adulthood, the flies are ready to mate and produce the next generation
of Drosophila melanogaster.

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During this experiment, monohybrid and dihybrid crosses were conducted
with Drosophila melanogaster. Our objective was to examine the inheritance from
one generation to the next. We collected the data from the crosses and analyzed
them in relation to the expected results.


MATERIALS AND METHODS
For the monohybrid cross in this experiment, we used an F1 generation, which
resulted from the mating of a male homozygous wild-type eyed fly with a female
homozygous sepia eyed fly. Males and females are distinguished by differences in
body shape and size. Males have a darker and rounder abdomen in comparison to
females, which are more pointed. Another difference occurs on the forelegs of
the fliesmales have a small bump called sex combs. At week 0, after being
anaesthitized by fly-nap, three males and three females were identified under a
dissecting microscope and placed in a plastic vial with a foam stopper at the
end. The vial remained on its side until the flies regained consciousness so
that they didnt get trapped by the culture medium at the bottom. We allowed
the Drosophila to incubate and reproduce for a week. After one week, the vial
contains many larva in addition to the F1 generation flies. Next, we removed
the F1 generation flies to prevent breeding between the two generations. Acting
as Dr. Kevorkian, we gave the F1 generation a lethal dose of the seemingly
harmless anesthesia, fly-nap. A trumpet solo of “Taps” played in our minds as
we said goodbye and placed them in the fly morgue. We allowed the F2 larval
generation to incubate for two weeks. The experiment called for one week of
incubation, but Easter fell during that week which interfered with our lab time.

After the two weeks, the F2 flies were also terminally anaesthetized. Only,
before saying goodbye, we separated the flies according to sex and eye
color(wild-type,red or mutant, sepia), recording the results in Table 1. The
same method was used it the dihybrid cross, except, instead of one trait, two
traits were observed. The traits were eye-color(wild-type, red or mutant,
sepia) and wing formation(wild-type, full or mutant, vestigial). The F1
generation for the dihybrid cross came from a cross between a male homozygous
wild-type for eyes and wings, and a female homozygous for sepia eyes and
vestigial wings. The results of this cross were recorded and appear in Table 2.


RESULTS
The monohybrid cross of Drosophila melanogaster produced 25,893 flies for all of
the sections combined. Of those flies, 75.9% had wild-type(red) eyes, and 24.1%
had mutant(sepia eyes). Overall, more females were produced than males.


TABLE 1: F1 Generation Monohybrid Cross of Drosophila melanogaster (+se x +se)
PHENOTYPECLASS RESULTS RESULTS
FROM ALL CLASSES NUMBER
PERCENT RATIO NUMBER PERCENT RATIO
MALES
WILD-TYPE EYES562 74.8% 3.0
8,96075.4% 3.1
SEPIA EYES189 25.2% 1 2,923
24.6%1
FEMALES
WILD-TYPE EYES806 75.6%3.1 10,685
76.3% 3.2
SEPIA EYES260 24.4%13,325
23.7% 1
BOTH SEXES
WILD-TYPE EYES1368 75.3% 3.019,645
75.9% 3.1
SEPIA EYES44924.7%1
6,248 24.1%1
The dihybrid cross produced a total of 26, 623 flies for all of the sections
combined. 54.9% of the flies had wild-type eyes(red) and wild-type wings(full),
17.7% had wild-type eyes and vestigial wings, 21.3% had sepia eyes and full
wings, and 6.1% had sepia eyes and vestigial wings. Again, the number of
females produced exceeded the number of males.


TABLE 2: F1 Generation Dihybrid Cross of Drosophila melanogaster(+vg+se x
+vg+se)
PHENOTYPE CLASS RESULTSRESULTS FROM ALL CLASSES
MALESNUMBER PERCENT RATIONUMBERPERCENT RATIO
WILD-TYPE EYESWILD-TYPE WINGS24447.8% 6.3
698754.4%8.6
WILD-TYPE EYESVESTIGIAL WINGS13225.9% 3.4
231518% 2.9
SEPIA EYESWILD-TYPE WINGS9518.6%2.4
272721.2%3.4
SEPIA EYESVESTIGIAL WINGS397.6%
18086.4%1 FEMALES
WILD-TYPE EYESWILD-TYPE WINGS281 51.1%7.0
761555.2%9.3
WILD-TYPE EYESVESTIGIAL WINGS100 18.2%
2.5239717.4%2.9
SEPIA EYESWILD-TYPE WINGS129 23.5%
3.2295321.4%3.6
SEPIA EYESVESTIGIAL WINGS407.3%
1821 6.0%1 BOTH SEXES
WILD-TYPE EYESWILD-TYPE WINGS52549.5%
6.614,60254.9% 9.0
WILD-TYPE EYESVESTIGIAL WINGS23221.9%
2.94,71217.7% 2.9
SEPIA EYESWILD-TYPE WINGS22421.1%
2.85,68021.3% 3.5
SEPIA EYESVESTIGIAL WINGS797.5%
11,6296.1%1
DISCUSSION
The results from the monohybrid cross for both my class and for all sections
were very close to the expected results.”Theoretically, there should be
three red-eyed flies for every one sepia-eyed fly. We call this a 3:1
phenotypic ratio” (So Whats a Monohybrid Cross Anyway? 2). As indicated in
table one, the data comes within one or two tenths of the 3:1 ratio. Therefore,
the monohybrid cross was very accurate. However, the results from the dihybrid
cross were not quite as accurate. Mendel hypothesized and proved that a
dihybrid cross should produce a 9:3:3:1 ratio(Biology 245). In our experiment,
the results from my class (both sexes) were not very close to the ratio. In
table 2, the ratio shows 6.6:2.9:2.8:1. The data obtained from all classes were
slightly more precise. All sections together (both sexes) produced a ratio of
9:2.9:3.5:1. There are many reasons that our results did not match the expected
ratios. For example, when transferring flies from one vial to another, a few
flies got away which could have a small effect on the numbers. Another factor
affecting the results also happened upon transferring flies. A number of flies
were imbedded in the cultural medium. We were forced to leave them there so
that we didnt loosen the medium. The largest source of error in the “my class”
column came from the amount of time we allowed the flies to reproduce. Since
Easter vacation occurred during our lab period, our second generation flies were
permitted to stay together for two weeks instead of one. This may have
resulted in the F2 generation flies mating with their own offspring, thus
throwing off the ratio. I feel more certain about the results in the “all
classes” column since many more trials were performed and more flies were used.

In any experiment, the more trials one conducts, the more accurate the results
will be.This makes sense when comparing the results from my class versus the
results from all classes combined. The numbers of flies used in each column
make the difference in trials more evident: 1,060 flies were produced in my
class, whereas 26, 623 flies were produced in all classes. In the monohybrid
cross, the ratio for eye color for the females were consistent with the ratio
for males. This information implies that the gene for eye color is not sex
linked. Through research, I found that in Drosophila melanogaster, chromosome
one is the sex chromosome. Eye color is not one chromosome one, but rather on
chromosome three. Therefore, eye color in Drosophila is not sex
linked(Genetics:Drosophila Crosses). In each column, the number of females
produced outweighed the number of males. This may imply that the X chromosome
is dominant over the Y chromosome. This would cause the X chromosome to mix
with another X chromosome, producing a female, more often than it would mix with
the Y chromosome, which would produce a male. As a follow-up to the experiment,
I would perform many more trials than each person did for this experiment. Also,
more flies could be placed in each vial to ensure even more offspring to be
included in the data. I would also be sure to remove the flies after just one
week to reduce breeding between generations. This experiment caused Mendels
findings to be more concrete and realistic in my mind. It made the information
more than meaningless numbers. The experiment also made me realize how easily
biological ideas can be proved. Our results agree with Mendels discoveries.

The only drawback to our learning was the massacre of over 26,000 fruit flies.


REFERENCES
Campbell, Neil A., Biology: Fourth Edition. Menlo Park: Benjamin/Cummings,
1996. “Genetics: Drosophila Crosses.” Lab Handouts, General Biology Lab, 1996.

“So Whats a Monohybrid Cross Anyway?” Lab Handouts, General Biology Lab, 1996.


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